Semen analysis is gold standard test to see the severity of male infertility.
Lowest normal reference value where 5% of population getting pregnancy is –
Count: 15 million
Motility (Forward progressive): 32%
Morphology: 4%
NA Fragmentation: 70 % (N) atleast
DNA Fragmentation index indicates beyond count & motility. It directly or indirectly indicate the power of sperm or ability of a sperm to fertile egg. It is quantitative & qualitative estimation of semen.
At WINGS we are doing SCD technique which is simple, less laborious& less expensive plus more sensitive.
Swim the sperm from a two density medium, so at the bottom we will get a best sperm morphologically. It will decrease 15% fragmentation.
Magnetic Activated Sell Sorting is a technique where double density gradient passing through a magnetic field, so apoptic sperm (which is in turn toward death program) will attach & non apoptic sperm will be down at the bottom.
We are doing MACS in
When we do ICSI, ultimate goal is selection of the scientifically smart sperm in an egg, means we need best DNA packaged sperm can be done by seeing the best sperm under 6000 resolution, It is the last scientific option. It is called IMSI, added advantage on ICSI,
We are doing at WINGS in
Intra-Cytoplasmic Sperm Injection (ICSI) is a process where a single sperm is injected directly into the egg using a fine glass needle. The main objective of ICSI is to ensure that the spermatozoa fertilize the egg membrane. The woman is stimulated with medications and then an egg retrieval procedure is performed so that several eggs can be obtained to attempt an ICSI procedure.
ICSI is recommended in cases of severe male infertility, previous fertilization failures with IVF or situations in which there are a limited number of oocytes available.
Steps to follow in an ICSI treatment
Precautions to take after an ICSI treatment